مجلة الاطروحة العلمية المحكمة الاطروحه للنشر العلمي
Streptomyces misionensis30في تشخيص المواد الحيوية الفعالة سطحيا المنتجة من سلالة GC-Massاستخدام تقنية
د. نادية حسين وعد الحيالي
كلية الحدباء الجامعة / قسم تقنيات المختبرات الطبية
أ.د. إسراء غانم حازم السماك
جامعة الموصل / كلية العلوم / قسم علوم حياة
Abstract
Fifty soil samples were collected from hydrocarbons contaminated and non-contaminated soils from Nineveh Government. Two isolates belongs to Streptomyces misionensiswhich from 8% from contaminated soil were diagnosed depending on the 16s rDNA to strains Streptomycesmisionensis 30 and Streptomycesmisionensis 31 compared to reference strain within the National Center Biotechnology Information site (NCBI). From 30 wounds and burns samples (33.3%) of Staphylococcus aureusand (6.6%) from Pseudomonas aeruginosawere isolated. Which showed multiple resistance to antibiotics, Oxacillin, Erythromycin, Nalidixic acid and Tetracycline. The Streptomyces misionensis30 strain showed ability to produce biosurfactant with antagonistic activity against methicillin resistant Staphylococcus aureusat a minimum inhibitory concentration 62.5 µg/cm3 as well as the fungus Aspergillusniger. The biosurfactant was diagnosed as Lipopeptide by using thin layer chromatography and GC-Mass technique and The surface efficacy of the biosurfactant extract is due to the presence of Silane derivatives.
Keyword: Streptomycesmisionensis, Biosurfactant, GC-Mass