مجلة الاطروحة العلمية المحكمة الاطروحه للنشر العلمي
تقييم بعض عوامل الضراوة والتحقيق الجزيئي للبيوفيلم في المبيضات البيض المعزولة من تجويف الفم
سوسن عبد الله احمد
بري لطيف محمد
ايمان تاجر عبد الله
قسم علوم الحياة / كلية العلوم / جامعة كركوك
Abstract
One hundred and twenty oral samples were collected from patients (with different ages and genders) of Azadi Teaching Hospital and some other private clinic in Kirkuk city from December 2019 to April 2020. The collected samples were identified based on conventional diagnostic methods (10% KOH and culture on different media). The virulence factors of isolated all Candida albicans such as hydrolytic enzymes and biofilm were also detected.Direct microscopic examination showed that (31.6%) was positive for Candida spp., while culturing on Sabouraud Dextrose Agar medium revealed (42.5%) Candida species. The highest percentage of oral candidiasis was in the age group less than one year (23.53%) & the lowest was in 31-60 years (15.68%). Females are more susceptible to oral Candidiasis (62.75%) than males (37.25%). Patients with cancer also revealed the highest rate of infection (31.37%), followed by (29.41%) of frequently antibiotics consumers, (15.69%) smokers and (11.76%) patients have diabetes mellitus type 2. The most detected Candida species was C. albicans 62.75% (32 of 51) followed by C.krusei (17.65%), C.tropicalis (9.8%), C. dubliniensis (7.84%) and the lowest isolated species was C.glabrata (1.96%).All Candida albicans showed proteinase and phospholipase activities, (78.13%) and (34.38%) of them were strong producers respectively. The (43.75%) of C. albicansisolates showed hemolysis on glucose -enriched SDA agar medium.Comparative study between microtiter plate (MTP) and Congo red agar (CRA) for biofilm formation in C. albicans showed that MTP is the most appropriate conventional method (9.38%) compared with CRA method (3.13%).On the other hand, molecular detection (PCR) of biofilm formation in 10 isolates showed that (90%) of isolates have ASL1 gene and (50%) have HWP1gene by using Polymerase chain reaction (PCR).
Key words:Candida albicans,Biofilm formation, oral candidiasis