مجلة الاطروحة العلمية المحكمة الاطروحه للنشر العلمي
تقييم تأثير فيتامين دي ثري وانترلوكين 10 في المرضى المصابين بالتهاب دواعم السن في صلاح الدين في العراق
امنه برير شكر
اياد مقداد غيدان
قسم علوم الحياة / كلية العلوم / جامعة تكريت
Abstract
Periodontal diseases refer to common inflammatory disorders, which are gingivitis and periodontitis, caused by a pathogenic microbiota in the subgingival biofilm that triggers inflammatory, innate, and adaptive immune responses. Aim of the study: Measuring the levels of IL_10 and VD3 for patients suffering from periodontitis, for people who use orthodontic treatment, and comparing them with healthy people who do not use orthodontic treatment . Methods: 90 blood samples were drawn using a sterile syringe with a capacity of 5 ml (60 blood samples from people with gingivitis) and (30 blood samples from healthy people in the control group). After that, the blood samples were separated by a centrifuge at a speed of 3500 rpm. For ten minutes separate the serum, then place the serum in a 1.5 ml Eppendorf tube and then store it at a temperature of -20°C until used for immunological tests .Results: The results recorded that Periodontitis infection distribution in age groups (10-20, 21-30 and > 31) were (65%, 28.3% and 6.7%), About (98.3%) showed positive bacterial growth which were Streptococcus pyogenes (61.9%), Staphylococcus aureus (11.1%), Enterococcus fecalis (7.9%), Streptococcus mutans (6.3%), Klebsiella spp and Staphylococcus epidermidis ( 4.8% %) for both, and (1.6 %) for Streptococcus sanguinis and kocuria kristinae. There was non-significant increase in IL – 10 level in patients with Periodontitis in comparison with control group that were (14.8±3.20, 10.9 ± 2.71), at p-value > 0.05.There was significant decrease in V D 3 level in patients with Periodontitis in comparison with control group at p-value > 0.05,Conclusion:The study’s findings indicate that there are no IL-10 level variations between the periodontitis patients and the control group. Patients with periodontitis had significantly higher V D 3 levels than the control group (p-value < 0.001).
Keywords : IL-10, V D 3, Periodontal diseases